Email updates

Keep up to date with the latest news and content from Veterinary Research and BioMed Central.

Open Access Research

Bovine HEXIM1 inhibits bovine immunodeficiency virus replication through regulating BTat-mediated transactivation

Hong-yan Guo1, Yong-gang Ma2, Yuan-ming Gai1, Zhi-bin Liang1, Jing Ma1, Yang Su1, Qi-cheng Zhang1, Qi-min Chen1 and Juan Tan1*

Author Affiliations

1 Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory Microbial Functional Genomics (Tianjin), College of Life Sciences, Nankai University, Tianjin, 300071, China

2 College of Pharmacy and Tianjian Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300071, China

For all author emails, please log on.

Veterinary Research 2013, 44:21  doi:10.1186/1297-9716-44-21

Published: 27 March 2013

Abstract

The bovine immunodeficiency virus (BIV) transactivator (BTat) recruits the bovine cyclin T1 (B-cyclin T1) to the LTR to facilitate the transcription of BIV. Here, we demonstrate that bovine hexamethylene bisacetamide (HMBA)-induced protein 1 (BHEXIM1) inhibits BTat-mediated BIV LTR transcription. The results of in vivo and in vitro assays show direct binding of BHEXIM1 to the B-cyclin T1. These results suggest that the repression arises from BHEXIM1-BTat competition for B-cyclin T1, which allows BHEXIM1 to displace BTat from B-cyclin T1. Furthermore, we found that the C-terminal region and the centrally located region of BHEXIM1 are required for BHEXIM1 to associate with B-cyclin T1. Knockdown of BHEXIM1 enhances BIV replication. Taken together, our study provides the first clear evidence that BHEXIM1 is involved in BIV replication through regulating BTat-mediated transactivation.