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Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells

Tufária Mussá1, Carolina Rodríguez-Cariño2, Alejandro Sánchez-Chardi3, Massimiliano Baratelli1, Mar Costa-Hurtado1, Lorenzo Fraile4, Javier Domínguez5, Virginia Aragon16 and María Montoya16*

Author Affiliations

1 Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain

2 Cátedra de Patología, Facultad de Ciencias Veterinarias, Universidad Central de Venezuela, Maracay, Venezuela

3 Servei de Microscopia, Facultat de Biociències, Campus UAB, 08193 Bellaterra, Barcelona, Spain

4 Universitat de Lleida, Lleida, Spain

5 Dpto. de Biotecnología, INIA, Madrid, Spain

6 Institut de Recerca i Tecnologia Agroalimentaria (IRTA), Barcelona, Spain

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Veterinary Research 2012, 43:80  doi:10.1186/1297-9716-43-80

Published: 16 November 2012

Abstract

Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.