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Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay

Clare FJ Grant13, Eric A Lefevre1, B Veronica Carr1, Helen Prentice1, Simon Gubbins2, Andrew J Pollard3, Catherine Charreyre4 and Bryan Charleston12*

Author Affiliations

1 Institute for Animal Health, Compton Laboratory, High Street, Compton, Berkshire, RG20 7NN, United Kingdom

2 Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey, GU24 0NF, United Kingdom

3 Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, OX3 9DU, United Kingdom

4 Merial Animal Health, SAS, Avenue Tony Garnier, Lyon, 69007, France

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Veterinary Research 2012, 43:68  doi:10.1186/1297-9716-43-68

Published: 10 October 2012

Abstract

Understanding the mechanisms that maintain protective antibody levels after immunisation is important for vaccine design. In this study, we have determined the kinetics of plasma and memory B cells detectable in the blood of cattle immunised with model T-dependent or T-independent antigens. Immunisation with the T-D antigen resulted in an expansion of TNP-specific plasma cells post-TNP primary and booster immunisations, which was associated with increased titres of TNP-specific IgG antibodies. Although no TNP-specific memory B cells were detected in the T-D group following the primary immunisation, we detected an increase in the number of TNP-specific memory B cells post-TNP boost. In contrast, no TNP-specific plasma or memory B cells were detected after primary or secondary immunisation with the T-I antigen. We then investigated if immunisation with a third party antigen (tetanus toxin fragment C, TTC) would result in a bystander stimulation and increase the number of TNP-specific plasma and memory B cells in the T-D and/or T-I group. TTC immunisation in the T-D group resulted in a small increase in the number of TNP-specific plasma cells post-TTC primary immunisation and boost, and in an increase in the number of TNP-specific memory B cells post-TTC boost. This bystander effect was not observed in the animals previously immunised with the T-I antigen. In conclusion, the present study characterised for the first time the B cell response in cattle to immunisation with T-D and T-I antigens and showed that bystander stimulation of an established T-D B cell memory response may occur in cattle.