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The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

Beatriz San Román1, Victoria Garrido1, Pilar-María Muñoz12, Laura Arribillaga3, Begoña García1, Ximena De Andrés1, Virginia Zabaleta1, Cristina Mansilla3, Inmaculada Farrán1, Iñigo Lasa1, Damián De Andrés1, Beatriz Amorena1, Juan-José Lasarte3 and María-Jesús Grilló1*

Author Affiliations

1 Instituto de Agrobiotecnología (CSIC-UPNA-Gobierno de Navarra), Carretera de Mutilva, s/n, 31192 Pamplona, Spain

2 Centro de Investigación y Tecnología Agroalimentaria (CITA) de Aragón (Gobierno de Aragón), Carretera de Montañana, 930, 50059 Zaragoza, Spain

3 Área de Terapia Génica y Hepatología, Centro de Investigación Médica Aplicada (CIMA), Pío XII, 55, 31008 Pamplona, Spain

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Veterinary Research 2012, 43:31  doi:10.1186/1297-9716-43-31

Published: 19 April 2012


The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking O-PS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella Enteritidis rough mutants, carrying either intact (SEΔwaaL) or deep-defective (SEΔgal) LPS-Core, were used in combination with EDA. Biotinylated bacterins, in particular SEΔwaaL bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from Salmonella Enteritidis lacking O-PS and the usefulness of BEDA-SEΔwaaL as non-live vaccine in the mouse model.