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Open Access Short report

Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes

Karlen Gazarian1*, Alvaro Aguilar Setién2, Tatiana Gazarian3 and Sebastian Aguilar Pierle4

Author Affiliations

1 Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), México D.F., Ciudad Universitaria, 04510, México

2 Unidad de Investigación Médica en Inmunología, Coordinación de Investigación IMSS Hospital de Pediatría 3er piso, Centro Medico Nacional Siglo XXI, Av. Cuauhtemoc 330, Doctores, México D. F., 06720 México

3 Facultad de Medicina, Universidad Nacional Autónoma de México, (UNAM), México D.F., Ciudad Universitaria, 04510, México

4 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, PO Box 647040 Pullman, WA, USA

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Veterinary Research 2011, 42:87  doi:10.1186/1297-9716-42-87

Published: 22 July 2011

Abstract

Using phage display and IgG of a goat infected with Caprine Arthritis Encephalitis Virus (CAEV) we obtained families of 7 mer constrained peptides with consensus motifs LxSDPF/Y and SWN/KHWSY and mapped the epitopes mimicked by them at the Env 6-LISDPY-11 and 67-WNTYHW-72 sites of the mature gp135 amino acid sequence. The first epitope fell into the N-terminal immunogenic aa1-EDYTLISDPYGFS- aa14 site identified previously with a synthetic peptide approach; the second epitope has not been described previously. The first epitope is mostly conserved across CAEV isolates whereas the second newly described epitope is extremely conserved in Small Ruminant Lentiviruses env sequences. As being immunodominant, the epitopes are candidate targets for mimotope-mediated diagnosis and/or neutralization.