Research
Molecular characterization of the uncultivatable hemotropic bacterium Mycoplasma haemofelis
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* Corresponding author: Emily N Barker emi.barker@bristol.ac.uk
- † Equal contributors
1 School of Veterinary Sciences, University of Bristol, Langford, BS40 5DU, UK
2 Centre for Genomic Research, Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK
3 Proteomics Facility, University of Bristol, Bristol, BS8 1TD, UK
4 School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK
5 School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
6 Institute of Infection and Global Health, University of Liverpool, Liverpool, L69 3BX, UK
Veterinary Research 2011, 42:83 doi:10.1186/1297-9716-42-83
Published: 12 July 2011Abstract
Mycoplasma haemofelis is a pathogenic feline hemoplasma. Despite its importance, little is known about its metabolic pathways or mechanism of pathogenicity due to it being uncultivatable. The recently sequenced M. haemofelis str. Langford 1 genome was analysed and compared to those of other available hemoplasma genomes.
Analysis showed that in hemoplasmas genes involved in carbohydrate metabolism are limited to enzymes of the glycolytic pathway, with glucose appearing to be the sole energy source. The majority of the pentose phosphate pathway enzymes that catalyze the de novo synthesis of ribonucleotides were absent, as were cell division protein FtsZ and chaperonins GroEL/ES. Uncharacterized protein paralogs containing putative surface expression motifs, comprised 62% of M. haemofelis and 19% of Mycoplasma suis genome coverage respectively, the majority of which were present in a small number of unstructured islands. Limited mass spectrometry and immunoblot data matched a number of characterized proteins and uncharacterized paralogs, confirming their expression and immunogenicity in vivo.
These data have allowed further characterization of these important pathogens, including their limited metabolic capabilities, which may contribute to their uncultivatable status. A number of immunogenic proteins, and a potential mechanism for host immune system evasion, have been identified.