Figure 4.

Overview of the genetic relationships between the two main identified genotypes of ISAV. Publically available ISAV sequences were imported into Bioedit version [76]and a multiple alignment performed using Clustal X [77]. Identical sequences were identified and only a single representative of each sequence type retained in the dataset to reduce subsequent analytical bias. The final alignment consisted of 38 unique sequences spanning a region of 987 (positions 25-1012 with respect to the open reading frame of ISAV segment 6). The phylogenetic relationship among ISAV isolates was inferred using the method described in the legend of Figure 2.

Snow Veterinary Research 2011 42:56   doi:10.1186/1297-9716-42-56
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