Overview of the genetic relationships between the main recognized genotypes of VHSV based on a dataset comprised of partial G gene data from 2 representatives of each of the established major genetic subgroups. Publicly available sequences derived from up to 2 isolates from each genotype recognized to date were imported into Bioedit version 188.8.131.52  and a multiple alignment performed using Clustal X . The final alignment consisted of 17 unique sequences spanning a partial region of the VHSV G Gene (649nt). The phylogenetic relationship among VHSV isolates was inferred using a maximum likelihood based approach implemented within PAUP* (version 4.0; ) and using the PaupUP interface v184.108.40.206 . The jModeltest 0.1.1 program ) was used to identify the model that best fits the sequence data from 56 models using the Akaike Information Criterion (AIC; ). The optimal unrooted maximum likelihood tree was identified using a heuristic search implemented in PAUP* and evaluated using 100 bootstrap iterations . Significant bootstrap values for the major clades were transferred to the unrooted tree derived from the original data.
Snow Veterinary Research 2011 42:56 doi:10.1186/1297-9716-42-56