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Table 1
Genes analysed and real time RT-PCR conditions
Genes	GenBank¹	Species²	Primers (5'→ 3')³	bp⁴	PCR conditions⁵

					Ta	[nM]	r²	Slope

HSP27	BT021550	Bovine	F: TGGCGCGTGTCCCTGGA	80	60	300	0.990	-3.33
			R: GTGATCTCCACCACGCC			300
HSP72	AY662497	Bovine	F: ACCCGCAGAACACGGTGTT	119	60	900	0.995	-3.33
			R: AGGCTTGTCTCCGTCGTTGA			900
HSP73	NM_174345	Bovine	F: CAACCTGCTTGGCAAGTTTGA	108	60	900	0.990	-3.20
			R: GAAACATTGAGGATGCCATTGG			900
HSP90	[30]	Ovine	F: AGTCTGGAGGATCCCCAGACA	78	60	300	0.994	-3.32
			R: GGGTCATCCTCGTCAATACCA			300

¹GenBank accession numbers of the sequences used for primer design. ²Species of origin of the sequences. ³Primers (F: Forward and R: Reverse) used for the gene amplification. ⁴Length of the amplicon in base pairs (bp). ⁵Real-time RT-PCR conditions for gene expression analyses: annealing temperature (Ta), primer concentration ([nM]), correlation coefficient (r²) and slope of the standard curve.
Serrano et al. Veterinary Research 2011 42:13 doi:10.1186/1297-9716-42-13

Table 1